PKC-induced Sensitization of Ca2+-dependent Exocytosis Is Mediated by Reducing the Ca2+ Cooperativity in Pituitary Gonadotropes

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PKC-induced Sensitization of Ca2+-dependent Exocytosis Is Mediated by Reducing the Ca2+ Cooperativity in Pituitary Gonadotropes

The highly cooperative nature of Ca2+-dependent exocytosis is very important for the precise regulation of transmitter release. It is not known whether the number of binding sites on the Ca2+ sensor can be modulated or not. We have previously reported that protein kinase C (PKC) activation sensitizes the Ca2+ sensor for exocytosis in pituitary gonadotropes. To further unravel the underlying mec...

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Cooperativity in Pituitary Gonadotropes

The highly cooperative nature of Ca 2 -dependent exocytosis is very important for the precise regulation of transmitter release. It is not known whether the number of binding sites on the Ca 2 sensor can be modulated or not. We have previously reported that protein kinase C (PKC) activation sensitizes the Ca 2 sensor for exocytosis in pituitary gonadotropes. To further unravel the underlying me...

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Exocytosis, dependent on Ca2+ release from Ca2+ stores, is regulated by Ca2+ microdomains.

The relationship between the cellular Ca2+ signal and secretory vesicle fusion (exocytosis) is a key determinant of the regulation of the kinetics and magnitude of the secretory response. Here, we have investigated secretion in cells where the exocytic response is controlled by Ca2+ release from intracellular Ca2+ stores. Using live-cell two-photon microscopy that simultaneously records Ca2+sig...

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Increased contractility of hepatic stellate cells in cirrhosis is mediated by enhanced Ca2+-dependent and Ca2+-sensitization pathways.

Activation of hepatic stellate cells (HSCs) results in cirrhosis and portal hypertension due to intrahepatic resistance. Activated HSCs increase their contraction after receptor agonist stimulation; however, the signaling pathways for the regulation of contraction are not fully understood. The aim of this study was to elucidate the change in contractile mechanisms of HSCs after cirrhotic activa...

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An Inhibitory Effect of Extracellular Ca2+ on Ca2+-Dependent Exocytosis

AIM Neurotransmitter release is elicited by an elevation of intracellular Ca(2+) concentration ([Ca(2+)](i)). The action potential triggers Ca(2+) influx through Ca(2+) channels which causes local changes of [Ca(2+)](i) for vesicle release. However, any direct role of extracellular Ca(2+) (besides Ca(2+) influx) on Ca(2+)-dependent exocytosis remains elusive. Here we set out to investigate this...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 2005

ISSN: 1540-7748,0022-1295

DOI: 10.1085/jgp.200409230